BILBO BETA

Bilayer gap is a buffer, not experimental The inter-leaflet half-gap (default 2.5 A per leaflet) prevents tail overlap in the template-tiled structure. It does not represent measured bilayer thickness. Adjust and re-minimize as needed.

Asymmetric bilayers When upper and lower leaflets differ in composition, check that the lipid area per molecule is compatible between leaflets. A large area mismatch produces curvature stress that persists even after minimization.

Lipid template names Template PDB filenames must match the lipid names entered above (case-insensitive). Upload your own templates via the CLI (bilbo membrane build --templates-dir) for lipids not included in the default set.

Atom count limit PDB serial numbers wrap at 99,999 atoms; residue numbers wrap at 9,999. BILBO handles this automatically, but downstream tools that rely on unique serial numbers should use the GRO file instead.